About...

PlanTherm PT100 device allows fast and easy estimation of heat stability of plants, algae or cyanobacteria. A leaf segment or algae cells are immersed in a water bath with de-iononized water and gradually heated from 20°C up to 85°C, duration approx. 30 minutes. The linear heating is coupled with a continuous monitoring of changes in the conductivity of the bath.

Key Features

Sample chamber for small up to middle site leaves, leaf segments, algae or cyanobacteria suspensions.
Precisely controlled gradual (linear) heating of plant segments with the slope up to 3 °C/min
Accurate temperature measurements (±0.02 °C) within the range of 20 – 85 °C
High accuracy conductivity measurement within 0.01 – 1,000 µS/cm

Measurement of the minimal chlorophyll ﬂuorescence intensity (F₀ level)
Compact device including measuring unit, magnetic stirrer, thermoregulator and mini computer in one case
Built-in scripting (R environment) language allows custom analysis of measured responses

PlanTherm PT 100

PlanTherm PT 100 device allows fast and easy estimation of heat stability of plants. A plant segment is immersed in a water bath with deionized water and gradually (linearly) heated (20 – 85 °C, duration approx. 30 minutes). During the heating, changes in the conductivity of the bath are continuously monitored.

The measured conductivity-temperature curve enables to determine the exact threshold temperature when leakage of ions from cells occurs. The threshold temperature is taken as a measure of the heat stability of plants. Simultaneous detection of chlorophyll a ﬂuorescence intensity (so called minimal ﬂuorescence level – F0) during the heating provides information on heat stability of primary photosynthetic processes in the plant material. PlanTherm device is designed for analysis of various plant material ranging from small up to middle size leaves or leaf segments.

PlanTherm PT 100 is provided with built-in Mini-PC unit with preinstalled Windows 10 operation system, which offers intelligent and intuitive user-interface. ProfleCon graphical user interface allows to setup experiment, analyze data and export measured curves to textual output fle. Plots of currently running experiment, measured raw data and analyzed results can be displayed on the main screen.

Applications

Fast estimation of both the constitutive and inducible heat tolerance of plants
Thermal stability of plants
Stress tolerance and acclimation responses
Photosynthetic research
Detection of biotic and abiotic stress
Plant’s resistance or susceptibility to stress factors
Agriculture and horticulture

Software

Preinstalled Windows 10 operation system
ProfleCon control and visualization software
R software environment for running the custom analysis on measured signals

Specifications

Measured Fluorescence Parameters:
F0, Fluorescence/Temperature curve with up to 4 ﬂuorescence critical points estimation

Measured Conductivity Parameters:
Conductivity/Temperature curve with conductivity critical point estimation

Fluorescence Module Excitation Light Sources:
460 nm for both the saturation light and FT measuring pulse; individual intensity settings

Protocols:
Custom defined linear heating (1 – 3 °C/min) in defined temperature range

Temperature Control Range:
20 – 85 °C

Water Bath Volume:
7 ml

Conductivity Measurement Resolution:
0.01 µS

Temperature Measurement Resolution:
0.01 °C

Stirrer Frequency:
0 – 2,000 RPM

Data Sampling Frequency:
2 Hz

Typical Samples:
Detached leaf segments of maximum size 0.5 × 2 cm

Analysis:
Predefined R script for critical point searching; analysis scripts free for custom modification

Mini PC Configuration:
Intel NUC Golden Lake D33217GKE i3-3217-U 1.8 GHz; min 90 GB SSD HD, 2 GB DDR3 RAM

Dimensions:
5 × 20 × 20 cm

Total Weight:
2 kg

Power Input:
Max. 70 W

Electrical:
90 – 240 V

Download

Documents

Plantherm User Guide (pdf)

Optional

Spare Cuvette for PlanTherm PT 100 *

Cuvette 402.000-OG 10mm

Sample Holder for PlanTherm PT 100

Includes sample holder and sample holder cube

Conductivity Probe for PlanTherm PT 100 *

PlanTherm sensor

Magnetic Stirrer Bar for PlanTherm PT 100

Magnetic stirrer bar PTFE 3x8 mm

Pricing

PlanTherm

Version	Characteristics	Price
PlanTherm PT 100	PT 100 is a complete system for fast and easy estimation of heat stability of plants.	9,990 €

Optional Accessories

Sample Holder	Includes sample holder and sample holder cube	90 €
Spare Cuvette *	Cuvette 402.000-OG 10mm	120 €
Conductivity Probe *	PlanTherm sensor	450 €
Magnetic Stirrer Bar for PlanTherm PT 100	Magnetic stirrer bar PTFE 3x8 mm	9 €